dba1 j mice  (Orient Bio Company)

Journal: Journal of Ginseng Research

Article Title: Effects of Korean Red ginseng on the regulation of inflammation, cell death, and fibrosis in a mouse model of rheumatoid arthritis featuring spike protein overexpression

doi: 10.1016/j.jgr.2026.101019

Figure Lengend Snippet: Effects of RGE on CIA in a mouse model of CIA injected with spike and ACE2 overexpression vectors. (A) Schematic outline of the in vivo experimental protocol. Male DBA1/J mice were immunized at the base of the tail with 100 μg of chicken type II collagen (CII) emulsified in complete Freund's adjuvant. A second dose, given 17 days later, used CII emulsified in incomplete Freund's adjuvant to establish the collagen-induced arthritis (CIA) model. Starting 7 days after the second immunization, mice received alternating intravenous injections of 200 μg pLEX307-ACE2 puro and 200 μg pcDNA3.1-SARS2-spike, each in 1 mL saline, at 7-day intervals. RGE (100 mg/kg) or MTX (3 mg/kg) was administered orally once per week from the same time point. All mice were sacrificed 7 weeks after the initial immunization. (B) Arthritis severity scores were evaluated in control, RGE-treated (n = 5), and MTX-treated (n = 5) groups. (C) Histopathological analysis of joint tissues using H&E staining was conducted to assess inflammation, cartilage destruction, and bone erosion. A total histological score was calculated for each group. (D) Treg cells, identified as Foxp3 + CD25 + cells, were visualized in splenocytes using confocal microscopy. (E ) Th17 cells, characterized as IL-17 + CD4 + T cells, were also detected in splenocytes via confocal imaging. Data were presented as mean ± SD. ∗ p < 0.05.

Article Snippet: Collagen-induced arthritis was induced in male DBA1/J mice (Orient Bio) as described by Lee et al. [ ].

Techniques: Injection, Over Expression, In Vivo, Adjuvant, Saline, Control, Staining, Confocal Microscopy, Imaging

Journal: Journal of Ginseng Research

Article Title: Effects of Korean Red ginseng on the regulation of inflammation, cell death, and fibrosis in a mouse model of rheumatoid arthritis featuring spike protein overexpression

doi: 10.1016/j.jgr.2026.101019

Figure Lengend Snippet: Effects of the combination of RGE and MTX on CIA in a mouse model of CIA injected with spike and ACE2 overexpression vectors. (A) Schematic representation of the in vivo experimental timeline. Male DBA1/J mice were immunized with chicken type II collagen (CII) emulsified in complete Freund's adjuvant (primary) and incomplete Freund's adjuvant (booster). Beginning 7 days after the second immunization, mice received alternating intravenous injections of 200 μg pLEX307-ACE2 puro and 200 μg pcDNA3.1-SARS2-spike (each in 1 mL saline) at 7-day intervals. RGE (100 mg/kg) in combination with MTX (3 mg/kg), or MTX alone, was administered orally once per week. All animals were sacrificed 7 weeks after the initial CII immunization. (B) Joint inflammation, bone erosion, and cartilage destruction were evaluated by H&E staining in the control, MTX, and RGE + MTX groups (n = 5 per group), and total histological scores were calculated. (C) Foxp3 + CD25 + regulatory T cells in splenocytes were visualized by confocal microscopy. (D) IL-17 + CD4 + T cells in synovial tissue were analyzed via confocal microscopy. (E) CD19 + IL-10 + B cells in splenocytes were detected by confocal imaging. ( F) Frequency of cells producing inflammatory cytokines including IL-17, IL-6, and MCP-1 in synovium as measured via IHC. Data were presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Collagen-induced arthritis was induced in male DBA1/J mice (Orient Bio) as described by Lee et al. [ ].

Techniques: Injection, Over Expression, In Vivo, Adjuvant, Saline, Staining, Control, Confocal Microscopy, Imaging